Part:BBa_K911010:Design

Bacillus shuttle vector (PJS130)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 7447
  Illegal XbaI site found at 3754
  Illegal XbaI site found at 7666
  Illegal XbaI site found at 7777
  Illegal PstI site found at 4552
  Illegal PstI site found at 6038
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 7447
  Illegal PstI site found at 4552
  Illegal PstI site found at 6038
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 7447
  Illegal BglII site found at 3737
  Illegal BglII site found at 7697
  Illegal BamHI site found at 7429
  Illegal XhoI site found at 4937
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 7447
  Illegal XbaI site found at 3754
  Illegal XbaI site found at 7666
  Illegal XbaI site found at 7777
  Illegal PstI site found at 4552
  Illegal PstI site found at 6038
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 7447
  Illegal XbaI site found at 3754
  Illegal XbaI site found at 7666
  Illegal XbaI site found at 7777
  Illegal PstI site found at 4552
  Illegal PstI site found at 6038
  Illegal NgoMIV site found at 2266
  Illegal NgoMIV site found at 4901
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 1303
  Illegal BsaI.rc site found at 5864
  Illegal SapI.rc site found at 4423
  

Design Notes

Bacillus shuttle vector. Includes pSPANK and b0010 terminator, both of which are integrated into our final construct.

Source

Designed and assembled by a member of our supporting lab, PJ steiner.